Leica Microsystems
Correlation of Fluorochromes and Filter Cubes
Fluorochrome Filtercube
Acid fuchsin
M2
N2.1
ACMA
E4
Acridine blue
A
Acridine orange
H3
I3
Acridine red
N2.1
N3
Acridine yellow
H3
I3
Acriflavin
E4
H3
Acriflavin-Feulgen-SITS (AFS)
D
Alexa 350
A4
Alexa 488
L5
Alexa 532
N3
Alexa 546
Y3
Alexa 555
Y3
Alexa 568
TX2
Alexa 594
Alexa 594
TX2
Alexa 633
Y5
Alexa 647
Y5
Alexa 700
Y7
Alizarin complexion
N2.1
Alizarin red
N2.1
Allophycocyanin (APC)
Y3
Y5
AMCA (Aminocoumarin)
A
AMCA/FITC/Texas Red
B/G/R
AmCyan
Aminoactinomycin D-AAD
N2.1
N3
Aniline blue
A
Astrazone Brilliant Red 4G
N2.1
Astrazone Orange R
I3
L5
Astrazone Red 6B
N2.1
Astrazone Yellow 7 CLL
H3
Atabrine
E4
H3
Auramine
H3
I3
Aurophosphine, Aurophosphine G
H3
I3
BCECF
FURA2
L5
Benzoxanthen Yellow
D
Berberine sulphate
H3
Beta-Lactamase
S Blue/Aqua
BFP/GFP (simultaneous)
BFP/GFP
BisAminophenyl Oxdiazol (BAO)
A
Bisbenzimide (Hoechst)
A
D
Blancophor BA
A
D
H3
Blancophor SV
A
BODIPY FL
I3
K3
L5
Brilliant Sulphaflavine FF
D
H3
Bromobimane (Thiolyte)
D
Calcein
I3
Calcein blue
A
Calcium Crimson
Alexa 594
Y3
Calcium Green
I3
K3
L5
Calcium Green-1
YFP HP
Calcium Orange
M2
N2.1
Calcofluor White
D
H3
Calcofluor White standard solution
A
Carboxyfluorescein diacetate C-FDA
I3
L5
Cascade Blue
A
D
Catecholamines (adrenalin, noradrenalin, dopa, dopamine)
D
CFP
CFP
CFP/YFP (simultaneous)
CFP/YFP
Chromomycin A (mithramycin, olivomycin)
E4
Coriphosphine O
H3
I3
Coumarin-phalloidin
D
Cy 2(TM)
GFP CY2
Cy 3
CY3/CY5
Y3
Cy 5
CY3/CY5
CY5
Y5
Cy 5.5
Y 5.5
Cy 7
Y7
Cy3/TRITC
CY3/TRITC
Cyan-Yellow-/Red-FP (simultaneous)
CFP/YFP/DsRed
CyQuant
GFP CY2
DANS (diamino-naphtyl sulphonic acid)
A
Dansyl chloride
A
DAPI
A
D
DAPI (selective)
A4
DAPI/FITC
B/G
DAPI/FITC/CY3
DAPI/FITC/CY3
DAPI/FITC/Texas Red (simultaneous)
B/G/R
DAPI/TRITC
B/R
DHE
DHE
Di-8-ANEPPS
DIA
DiI
Y3
DiO
GFP CY2
I3
K3
Diphenyl brilliant flavine 7 GFF
H3
DIPI
A
Dopamine
A
DPH (diphenyl hextariene)
A
EGFP
Alexa 594
GFP CY2
Eosin B
N2.1
YFP HP
Ethidium bromide
N2.1
Euchrysin
D
H3
Evans Blue
N2.1
Fast Blue
A
Fast Green FC G
M2
N2.1
FDA (fluorescein diacetate)
H3
I3
K3
L5
Feulgen
N2.1
TX2
FIF (formaldehyde induced fluorescence)
D
A
FITC (fluorescein isothiocyanate)
H3
I3
K3
L5
FITC (selective)
L5
FITC/CY3/CY5
FITC/CY3/CY5
FITC/ethidium bromide
I3
L5
N2.1
FITC/phycoerithrin (PE) (simultaneous)
G/R
FITC/Texas Red (simultaneous)
FITC/Texas Red
G/R
FITC/TRITC (simultaneous)
FI/RH
Fluo 3
I3
L5
YFP HP
Fluoro Gold
A
Fluram (fluorescamine)
A
FURA2
FURA2
Genacryl Brilliant Red B
N2.1
Genacryl Brilliant Yellow
E4
Generic Blue
D
GFP (Green Fluorescent Protein)
GFP
GFP CY2
GFP (Narrowband)
Granular Blue
A
Haematoporphyrin
N2.1
HcRed
Hoechst dye no. 33258
A
A4
D
Hoechst dye no. 33342
A
A4
D
Hydroxy-4-methylcoumarin
A
Indocyanine green
IRDye800
JP1
JP1
JP2
JP2
JRed
Keima
Lissamine-rhodamine B (RB 200)
M2
N2.1
Lucifer Yellow
E4
Magdala Red
N2.1
Maleimide
A
Mepacrin
D
Merocyanin 540
N2.1
Mithramycin
E4
MitoSOX red
MPS (methyl Green Pyronine stilbene)
A
mTFP
Nile Red
TX2
Nuclear Fast Red
M2
N2.1
N3
Nuclear Yellow
A
Olivomycin
E4
Oregon Green (488, 500, 514)
L5
Oxytetracycline
D
Pararosaniline (Feulgen)
N2.1
TX2
Phosphine 3 R
H3
I3
Phtalocyanine
Phycoerythrin (PE)
N2.1
N3
Primulin O
D
Procion Yellow
D
E4
H3
Propidium iodide
N2.1
Pyronine B
M2
N2.1
Qdot 525
Qdot 585
Qdot 625
Qdot605
Qdot 605
Quinacrine mustard (QM)
E4
Resorufin
N2.1
Y3
Reverine
D
RFP
Red GFP
Rhodamine 123
I3
L5
YFP HP
Rhodamine B
N2.1
RS-GFP (Narrowband)
Alexa 594
Serotin
A
D
sgGFP (TM)
GFP CY2
SITS acriflavine Feulgen
D
SITS (stilbene isothiosulphonic acid)
A
Spectrum Aqua
S Aqua
Spectrum Blue
S Blue
Spectrum Blue/Aqua
S Blue/Aqua
Spectrum FRED
S FRED
Spectrum Gold
S Gold
Spectrum Green
S Green
Spectrum Orange
M2
N2.1
S Orange
Spectrum Red
S Red
Sulphaflavine
A
SZ green
tdTomato
Tetracyclines: oxytetracycline, tetracycline, reverine (pyrrolidinomethyltetracycline), chlortetracycline, dimethylchlortetracycline
D
Texas Red
TX2
Texas Red (selective)
TX2
Thiazin red R
M2
N2.1
Thioflavine S
D
H3
Thioflavine TCN
A
Thiolyte (bromobimane)
D
A
TRITC (selective)
N3
TRITC (selective)
N3
TRITC (tetramethyl rhodamin isothiocyanate)
N2.1
N3
True Blue
A
TurboFP635
Uranine B
H3
Uvitex 2 B
A
D
wtGFP (non UV)
GFP CY2
XenoFluor 680
XRITC
N2.1
N3
Xylene orange
M2
N2.1
YFP
YFP
YFP HP
YO-PRO-1
GFP CY2
YOYO-1
GFP CY2
ZsYellow